Revvity Atplite 1step Luminescence Assay System, 100 Ml

ATP is a marker for cell viability due to its presence in all metabolically active cells. ATP concentration declines rapidly when cells undergo necrosis or apoptosis, and so monitoring ATP is a good indicator of cytotoxic, cytostatic and proliferation effects.

Our ATPlite 1step ATP luminescence assays use patented technologies to measure cell proliferation and cytotoxicity in mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.

Features and benefits:

Simple and reproducible: only one reagent addition step, no separation stepsSuitable for both 96- and 384-well microplatesLinear correlation between cell number and luminescent signal: up to 50,000 cells per well for 96-well microplates and 12,500 cells per well for 384-well microplatesDesigned for continuous process systems: luminescence should be measured between 0 and 30 minutes after reagent addition and plate shakingHomogeneous: no cell harvesting or centrifugation requiredHigh sensitivity: able to detect down on one cell per wellReduced Phenol Red dependencyHigh light-output: assay can be used with less sensitive luminescence readers such as multi-label readersFast: no luminescence signal stabilization time required

An alternative format of this assay is also available. The ATPlite assay has separate cell lysis and luminescent signal generation steps. This allows for a more stable signal, with half-life of at least 4 hours.