Revvity Ifn- (M) Lance Ultra Tr-

Revvity The LANCE Ultra Mouse IFN-γ Detection Kit is designed for detection and quantitation of mouse interferon γ in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity The 500 point kit contains enough reagents to run 500 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). The 10,000 point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 µL reaction volume (15 µL of sample). - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

No-wash steps, no separation stepsTR-FRET technologySensitive detectionHigh reproducibilityFaster time-to-resultsEasy automation96-well, 384-well, and 1536-well formats

Revvity LANCE and LANCE (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight^™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity In the mouse, Interferon-gamma (IFN-γ) is a homodimeric glycoprotein of 133 amino acids. Interferons (IFNs) were discovered due to their antiviral effects. In humans, there are three families of IFNs: IFN type I (IFN-α, ß, ω, ε, and κ), IFN type II (one single representative, IFN-γ), and IFN type III (IFN-λ1-3). Antigens and mitogens stimulate the production of IFN-γ in Natural Killer and activated helper T lymphocytes. IFN-γ shows multiple effects. It induces the production of cytokines, upregulates the expression of class I and II MHC antigens and leukocyte adhesion molecules. It also activates macrophages and enhances the secretion of immunoglobulins by B cells. Response to IFN-γ is mediated by the heterodimeric IFN-γ receptor. Importantly, IFNs have proven to be effective in the treatment of several viral infections and cancers. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)