Revvity The Alpha Surefire® Ultra™ Multiplex P53

The Alpha SureFire Ultra Multiplex p-p53 + Total p53 assay kit is used to measure both phosphorylated and total levels of endogenous p53 in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated p53 analysis, and the 545 nm (Tb) signal corresponds to the total p53 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies.

No-wash steps, no separation stepsMultiplexing assaySensitive detectionNo interference from exogenous antibodiesResults in less than 3 hours

In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody.