Revvity Alphalisa Surefire Ultra Multiplex Human Phospho

Revvity Anaplastic lymphoma kinase (ALK) also known as ALK tyrosine kinase receptor or CD246 (cluster of differentiation 246) is an enzyme that in humans is encoded by the ALK gene. ALK plays a pivotal role in cellular communication and in the normal development and function of the nervous system. The ALK gene can be oncogenic by forming a fusion gene with any of several other genes, by gaining additional gene copies or with mutations of the actual DNA code for the gene itself. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity The AlphaLISA SureFire Ultra Multiplex Human Phospho-ALK (Tyr1604)/Total ALK Detection Kit is used to measure both phosphorylated and total levels of endogenous ALK in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The 615 nm (Eu) signal corresponds to the phosphorylated ALK analysis, and the 545 nm (Tb) signal corresponds to the total ALK analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

No-wash steps, no separation stepsMultiplexing assaySensitive detectionNo interference from exogenous antibodiesResults in less than 3 hours

Revvity In this assay, the Alpha 615 Acceptor bead is coated with the CaptSure™ antibody, which binds the CaptSure-tagged anti-phospho target antibody. The Alpha 545 Acceptor bead is coated with the CaptSure2™ agent, which binds the CaptSure2 tagged anti-total target protein antibody. The Alpha Donor bead binds the biotinylated anti-total target protein antibody. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)