Revvity AlphaLISA AAV5 Capsid Detection Kit, 500 Assay

Revvity Adeno-associated virus (AA) vectors are the leading platform for gene delivery for the treatment of a variety of human diseases. AAV Serotype 5 is used as a vector for gene therapy of hemophilia A and diseases associated with its natural tissue tropism such as lung, retina, or central nervous system cells. The AAV5 kit is designed to detect and quantify AAV5 particles. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Formats

Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 50 µL reaction volume (10 µL of sample).Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (10 µL of sample).Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (10 µL of sample).

Features

No-wash steps, no separation stepsELISA alternative technologySensitive detectionBroad sample compatibilitySmall sample volumeResults in less than 3 hoursHalf the time of an ELISA assay

Revvity AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is captured by AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)