Revvity PhenoVe Fra-2 AM, Calcim Indicator

Revvity Calcium ions are important signaling molecules mediating a broad spectrum of intracellular, intercellular as well as extracellular functions. Ca²⁺ plays critical physiological functions such as muscle contraction, neuronal excitability, cell migration and cell growth. Intracellular Ca²⁺ is stored in the endoplasmic reticulum, sarcoplasmic reticulum, and the mitochondria. Upon cell surface receptors stimulation like GPCRs, calcium is released from these internal stores and its intracellular concentration increases from 100 nM to approximately 1 μM. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity Intracellular Ca²⁺ responses have been widely investigated with fluorescent calcium probes which fluorescence signal increases upon Ca²⁺ binding. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity The first-generation of fluorescent calcium indicators, including Fura-2, Fluo-3 and Rhod-2, were developed, followed by a second-generation, such as Fluo-4, with improved properties especially brightness and signal-to-noise ratio. However, the presence of anion transporters expressed at the cell surface may impair the intracellular retention of the fluorescent calcium indicators which are cleared out from cells. Inhibitors of these transporters, such as Probenecid, are commonly used but suffer from limitations such as cytotoxicity, off-targets, or inconsistent calcium flux kinetics. Such limitations can be overcome with third generation of fluorescent calcium indicators including Cal-520, Cal-590 and their corresponding Cal-520, Cal-590 Bright versions which do not require probenecid. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity PhenoVue Fluorescent Calcium Indicators, encompassing Fura-2 AM, Fluo-4 AM, Cal-520 AM, Cal-590 AM, Cal-520 AM Bright and Cal-590 AM Bright, enable a myriad of applications as well as multiplexing capability to unravel intracellular calcium mediated functions. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity PhenoVue Fura-2 AM is a high affinity cell permeable Ca²⁺ indicator which is rapidly metabolized by intracellular esterase, leading to a green, fluorescent signal. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity Features: - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Numbers of Vials Per Unit: 20Quantity or Volume Per Vial: 50 µg (50 nmoles)Form: SolidStorage: -16 °CRecommended Working Concentration: 5 µMMaximum Excitation Wavelength: 336 nm (Ca²⁺ free) / 365 nm (Ca²⁺ bound)Maximum Emission Wavelength: 505 nmLive Cell Staining: YesFixed Cell Staining: No

Revvity Filter Set: - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Ex/Em1 = 340/510 nm cutoff 475 nmEx/Em2 = 380/510 nm cutoff 475 nm

Revvity Equivalent Number of Microplates: - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

6-20 x 96-well microplates6-20 x 384-well microplates8-32 x 1536-well microplates