Revvity Phospho-Zap-70 (Tyr319) Lance Ultra Tr-Fret Det

Revvity Please note control lysates are sold separately, catalog number TRF4024S. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity Formats: - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Our 500 assay point kit allows you to run 500 wells in 384-well format, using a 20 µL reaction volume. Our 10,000 assay point kit allows you to run 10,000 wells in 384-well format, using a 20 µL reaction volume.

Revvity LANCE^® and LANCE^® (Lanthanide chelate excite) Ultra are homogeneous (no wash) TR-FRET (time-resolved fluorescence resonance energy transfer) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second antibody is labeled with an acceptor fluorophore [ULight™ dye]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm. Data are represented as ratiometric (665/615 nm X 10,000). - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity ZAP-70 (Zeta-chain-associated protein kinase 70) is normally expressed near the surface membrane of T-cells and natural killer cells, but levels in B-cells can be an indicator for various forms of leukemia. ZAP-70 is recruited to the TCR/CD3 complex in order to be phosphorylated. Once phosphorylated, ZAP-70 promotes downstream phosphorylation of proteins such as SLP76 and ERK. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)