Revvity Atplite 3d, 300 Assay Point Kit

Revvity ATP is a marker for cell viability because it is present in all metabolically active cells. Because ATP concentration declines rapidly when cells undergo necrosis or apoptosis, monitoring ATP is a good indicator of cytocidal, cytostatic and proliferation effects. ATP assay is key to successfully detect cell viability. Our ATPlite 3D™ and ATPlite 1step 3D luminescence assay systems use patented innovative technologies that measure cell proliferation and cytotoxicity in 3D cultured mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity ATPlite 3D is an Adenosine TriPhosphate (ATP) monitoring system based on firefly (Photinus pyralis) luciferase. This ATPlite luminescence assay is the alternative to colorimetric, fluorometric, high content microscopic analysis and radioisotopic assays for the quantitative evaluation of proliferation and cytotoxicity of mammalian cells cultured in 3D spheroids. ATP monitoring can be used to assess the cytocidal, cytostatic and proliferative effects of a wide range of drugs, biological response modifiers and biological compounds. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity Advantages: - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Long-lived luminescent signal: half-life (t1/2) greater than 5 hours, depending on cell type and mediumThe separate lysis step, using sodium hydroxide for tissue lysis, is the best option for full spheroid lysis and extraction of ATPRapid: results generated in 40 - 45 minutesSimple and reproducible: no separation steps; only two reagent additions and mixing. A final transfer step may be necessary, depending on the type of plate used for Spheroid growthHomogeneous assay: no cell harvesting or centrifugation requiredWide linear dynamic range: detect ATP from spheroids made by seeding as few as 200 cells, and up to 5000 cellsConvenient storage conditions (2 - 8 °C)No DTT: Unlike most luciferase detection reagents, lites products do not contain DTT, eliminating toxic hazards, unpleasant odors and the need for hood workExcellent Z Values: All lites products offer robust, sensitive performance, with high signal-to-background ratios and excellent Zvalues

Revvity Kit Components - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

3 vials of Lyophilized Substrate Solution1 x 20 mL of Substrate Buffer Solution1 x 20 mL of Mammalian Cell Lysis Solution (MCLS) 1 vial of Lyophilized ATP Standard Solution1 x CellCarrier Spheroid ULA 96-well microplate (product # 6055330; 10 plates, 6055334; 40 plates) 1 x OptiPlate-96 (product# 60052900 for 50 plates; 6005299 for 200 plates)4 x TopSeal-A (product # 6050185; 100 seals) 1 x Quick Start Guide

Revvity Storage: Buffer, MCLS and vials should be stored at 4°C. Microplates and TopSeal-A can be stored at Room Temperature. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)