Revvity AlphaLISA SureFire Ultra Human and Mouse Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Detection Kit, 50,000 Assay Points

Ezrin, Radixin, and Moesin (ERM) proteins link the plasma membrane to the actin cytoskeleton, maintaining cell shape and facilitating adhesion and motility. These proteins are activated through phosphorylation, which stabilizes their open conformation, enabling interaction with the cytoskeleton. Overexpression of ERM proteins is observed in cancers such as breast and pancreatic cancer, enhancing metastatic potential. Dysregulated ERM proteins also contribute to autoimmune disorders like multiple sclerosis.

The AlphaLISA SureFire Ultra Human and Mouse Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-Ezrin/Radixin/Moesin in cellular lysates, using Alpha Technology.

Formats:

The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

Cell and tissue lysatesAntibody modulatorsBiotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

Cellular kinase assaysReceptor activation studiesHigh-throughput screening for preclinical studies - REVPFZ (Additional S&H or Hazmat Fees May Apply)