Revvity Tau (H) Alphalisa Det.Ki

Revvity Formats: - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Revvity Features: - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

No-wash steps, no separation stepsELISA alternative technologySensitive detectionBroad sample compatibilitySmall sample volumeResults in less than 3 hoursHalf the time of an ELISA assay

Revvity Microtubule-associated Protein Tau (TAU) is a neuronal protein that binds to axonal microtubules and has roles in the assembly of microtubules, cytoskeletal structure, and axonal transport. Six isoforms of Tau have been described, ranging from 352 to 441 residues, all originating from the alternative splicing of one gene designated MAPT. Tau interacts with tubulin to stabilize the microtubules, but its phosphorylation blocks this association, resulting in disruption of microtubule organization. In humans, hyperphosphorylated Tau can self-assemble into very insoluble aggregates (paired helical filaments) leading to various pathologies. Tauopathy is the name given to diseases associated with Tau accumulation, among which Alzheimer’s disease (AD) is one of the best known. Tau is a biochemical marker for this neurodegenerative disease as an increase of Tau in cerebrospinal fluid is observed in most patients with AD. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)