Revvity Leptin (Mouse) Alphalisa

Revvity Formats: - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Revvity Features: - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

No-wash steps, no separation stepsELISA alternative technologySensitive detectionBroad sample compatibilitySmall sample volumeResults in less than 3 hoursHalf the time of an ELISA assay

Revvity In the mouse, leptin is a hormone that contains 146 amino acid residues and is secreted by differentiated adipocytes. It regulates energy homeostasis as a result of its action on the brain via hypothalamic neuronal pathways expressing the leptin receptor (LR). Hereditary deficiency of leptin, or functional LRs, causes severe obesity in humans and mice. Leptin-mediated signaling has been implicated in the regulation of food intake, energy expenditure, lipid and carbohydrate metabolism, and reproductive, neuroendocrine, thyroid, and immune function. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)