Revvity HTRF Human/Mouse Phospho-CHK1 Ser345 Detection

This HTRF cell-based assay enables the rapid, quantitative detection of CHK1 phosphorylated at Serine 345, as a readout of the ATR/CHK1 signaling pathway upon a DNA damage response (DDR)

In response to DNA damage, for example Single Strand Breaks (SSBs), the ATR-Chk1 pathway and replication checkpoint responses are activated, mediating G2/M checkpoints to arrest cell cycle progression and allow extra time for DNA repair. ATR is recruited to track of ssDNA-RPA through its interacting partner, ATRIP, which leads to Chk1 phosphorylation and activation and in turn induces downstream signaling.