Revvity HTRF Human Total BRD7 Detection Kit

The Total BRD7 cellular assay monitors BRD7 protein levels.

Bromodomain containing 7 (BRD7), also known as NAG4, BP75 or CELTIX1, is a member of the bromodomain-containing protein family IV, which also includes BRD9 protein. BRD7 is mainly localized in the nucleus and regulates chromatin remodeling. BRD7 is a component of a large polybromo-BAF (PBAF) complex, including ARID2, PBRM1, and BAF45d, which interacts with transcriptional regulators to modulate gene expression.

More importantly, recent studies have shown that BRD7 is downregulated in certain cancers such as epithelial ovarian carcinoma, breast cancer, nasopharyngeal cancer, and colorectal carcinoma. Unlike prooncogenic BRD9 roles, BRD7 may function as a tumor suppressor by inhibiting the Phosphoinositide 3-kinase (PI3K) pathway involved in various deregulated oncogenic processes, such as apoptosis, protein synthesis, proliferation, and angiogenesis.

Since BRD7 and BRD9 proteins share a 72% residue similarity, compounds are likely to target both the tumor suppressor BRD7 and the prooncogene BRD9. Therefore, the selectivity of compounds targeting either BRD9 or BRD7 must be carefully investigated. To do this, the HTRF total BRD7 kit is a valuable companion assay for the HTRF total BRD9 kit, enabling reliable compound profiling.