Revvity Alphalisa Surefire Ultra Total Smad3 Assay Kit

Revvity The AlphaLISA SureFire Ultra total SMAD3 assay is a sandwich immunoassay for quantitative detection of SMAD3 (phosphorylated and non-phosphorylated) in cellular lysates using Alpha Technology. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume. The 500 point kit contains reagents to run 500 wells in 384-well format, using a 20 μL reaction volume. The 10,000 point kit contains reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume. The 50,000 point kit contains reagents to run 500 wells in 384-well format, using a 20 μL reaction volume. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity In the AlphaLISA SureFire Ultra assay, Donor Beads are coated with streptavidin to capture one of the, which is biotinylated. Acceptor Beads are coated with a proprietary CaptSure^TM agent that immobilizes the other antibody, labeled with a CaptSure tag. In the presence of target protein, the two antibodies bring the Donor and Acceptor beads close together, generating signal. The amount of light emission is directly proportional to the amount of protein present in the sample. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity AlphaLISA SureFire Ultra kits are compatible with: - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Cell and tissue lysates Antibody modulators Biotherapeutic antibodies

Revvity Alpha SureFire kits can be used for: - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Cellular kinase assays Receptor activation studies Screening