Revvity AlphaLISA SureFire Ultra Human Total MYD88 Detection Kit, 100 Assay Points

Toll-like or interleukin-1 receptors (TLR/IL-1R) signaling is mediated by Myeloid Differentiation Factor 88 (MYD88), a crucial adapter that mediates the onset of the innate immune response and the generation of proinflammatory cytokines that inhibit pathogens and activate adaptive immunity. While a hosts ability to fend off pathogenic infection depends on MYD88, disruption of its abundance can result in autoimmune disorders. One important mechanism for stopping the production of cytokines is the degradation of MYD88.

The AlphaLISA SureFire Ultra Human Total MYD88 Detection Kit is a sandwich immunoassay for the quantitative detection of total MYD88 in cellular lysates, using Alpha technology.

Formats:

The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

Cell and tissue lysatesAntibody modulatorsBiotherapeutic antibodies

Alpha SureFire kits can be used for:

Cellular kinase assaysReceptor activation studiesHigh-throughput drug screening