Revvity AlphaLISA SureFire Ultra Human Phospho-HSP27

Revvity Heat shock protein 27 (Hsp27) is a protein that in humans is encoded by the HSPB1 gene. Hsp27 is a multidimensional protein which acts as a protein chaperone and an antioxidant and plays a role in the inhibition of apoptosis and actin cytoskeletal remodeling. In each of these capacities, HSP27 has been implicated in different disease states playing both protective and counter-protective roles. This protein has been indicated in renal injury, fibrosis, cancer, neurodegenerative disease, and cardiovascular disease. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity The AlphaLISA SureFire Human Phospho-HSP27 (Ser15) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-HSP27 (Ser15) in cellular lysates, using Alpha Technology. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Formats

The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

Cell and tissue lysatesAntibody modulatorsBiotherapeutic antibodies

Alpha SureFire kits can be used for

Cellular kinase assaysReceptor activation studiesScreening