Revvity AlphaLISA SureFire Ultra Human and Mouse Total PRAS40 Detection Kit, 100 Assay Points

Proline-rich AKT substrate 40 kDa (PRAS40) is a substrate of AKT kinase and a component of the mechanistic target of rapamycin complex 1 (mTORC1). Phosphorylation of PRAS40 by AKT relieves its inhibitory effect on mTORC1, thereby promoting cell growth and proliferation. Dysregulation of PRAS40 phosphorylation is implicated in various cancers and metabolic disorders, highlighting its role in cell survival and metabolism.

The AlphaLISA SureFire Ultra Human and Mouse Total PRAS40 Detection Kit is a sandwich immunoassay for the quantitative detection of total PRAS40 in cellular lysates, using Alpha Technology.

Formats:

The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

Cell and tissue lysatesAntibody modulatorsBiotherapeutic antibodies

Alpha SureFire Ultra kits can be used for:

Cellular kinase assaysReceptor activation studiesScreening