Revvity AlphaLISA Surefire Ultra Total p300 Detection Kit, 10,000 Assay Points

Revvity Histone acetyltransferase p300 is an acetyl transferase encoded by the EP300 gene with numerous aliases: p300, p300 HAT, E1A-associated protein p300 (where E1A = adenovirus early region 1A), and EP300. p300 mediates histone 3 lysine 27 acetylation (H3K27ac) at regulatory elements such as enhancers and promoters. p300 is over-expressed in cancer cells and drug-resistant cancer cells, it activates oncogene transcription and induces cancer cell proliferation, survival, tumorigenesis, metastasis, immune evasion, and drug-resistance. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity The AlphaLISA SureFire Human Total p300 Detection Kit is a sandwich immunoassay for the quantitative detection of total p300 in cellular lysates, using Alpha Technology. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Formats

The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

Cell and tissue lysatesAntibody modulatorsBiotherapeutic antibodies

Alpha SureFire kits can be used for

Cellular kinase assaysReceptor activation studiesScreening