Revvity AlphaLISA SureFire Ultra Mouse Phospho-RIPK1 (Ser321) Detection Kit, 10,000 Assay Points

Receptor-interacting serine/threonine-protein kinase 1 (RIPK1) is a critical regulator of cell death and inflammation. RIPK1 can mediate cell survival, apoptosis, or necroptosis depending on cellular context and post-translational modifications. In its active form, RIPK1 promotes necroptosis by interacting with RIPK3 and MLKL. Dysregulation of RIPK1 activity is implicated in inflammatory and neurodegenerative diseases, as well as in cancer.

The AlphaLISA SureFire Ultra Mouse Phospho-RIPK1 (Ser321) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-RIPK1 (Ser321) in cellular lysates, using Alpha Technology.

Formats:

The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

Cell and tissue lysatesAntibody modulatorsBiotherapeutic antibodies

Alpha SureFire Ultra kits can be used for:

Cellular kinase assaysReceptor activation studiesScreening