Revvity AlphaLISA Surefire Ultra Total TFEB Detection Kit, 100 Assay Points

Revvity Transcription factor EB (TFEB) plays a key role in the regulation of basic cellular processes, including lysosomal biogenesis and autophagy. Dephosphorylated TFEB travels to the nucleus to trigger the transcription of target genes while phosphorylated TFEB exists in the cytoplasm. Mechanistic target of rapamycin (mTOR)-mediated phosphorylation, which takes place at the lysosomal surface, controls the subcellular location and function of TFEB. TFEB is implicated in various diseases, such as lysosomal storage disorders, neurodegenerative diseases, cancers, and metabolic disorders. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity The AlphaLISA SureFire Human Total TFEB Detection Kit is a sandwich immunoassay for the quantitative detection of total TFEB in cellular lysates, using Alpha Technology. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Formats

The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

Cell and tissue lysatesAntibody modulatorsBiotherapeutic antibodies

Alpha SureFire kits can be used for

Cellular kinase assaysReceptor activation studiesScreening