Revvity AlphaLISA SureFire Ultra High Performance Human and Mouse Phospho-STAT1 (Tyr701) Detection Kit, 10,000 Assay Points

Signal Transducer and Activator of Transcription 1 (STAT1) is a pivotal transcription factor within the JAK/STAT signaling pathway, activated by interferons and various cytokines. Upon activation, STAT1 dimerizes and translocates to the nucleus, where it binds to DNA and regulates the expression of genes involved in immune defense and tumor suppression. Dysregulation of STAT1 can promote or inhibit tumor growth, drive chronic inflammation in autoimmune diseases like lupus and rheumatoid arthritis, and contribute to neuronal damage in neurodegenerative diseases.

The AlphaLISA SureFire Ultra High Performance Human and Mouse Phospho-STAT1 (Tyr701) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-STAT1 in cellular lysates, using Alpha Technology.

Formats:

The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

Cell and tissue lysatesAntibody modulatorsBiotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

Cellular kinase assaysReceptor activation studiesHigh-throughput screening for preclinical studies - REVPFZ (Additional S&H or Hazmat Fees May Apply)