Revvity AlphaLISA SureFire Ultra Human Total PAX5 Detection Kit, 10,000 Assay Points

PAX5 is a transcription factor crucial for B-cell development, regulating genes specific to B-cell identity. It interacts with transcription factors like EBF1 and RUNX1 to control gene expression during B-cell differentiation. Alterations in PAX5, including mutations and translocations, are implicated in B-cell acute lymphoblastic leukemia and non-Hodgkin lymphoma, disrupting normal B-cell function and driving malignancy.

The AlphaLISA SureFire Ultra Human Total PAX5 Detection Kit is a sandwich immunoassay for the quantitative detection of total PAX5 in cellular lysates, using Alpha Technology.

Formats:

The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

Cell and tissue lysatesAntibody modulatorsBiotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

Cellular kinase assaysReceptor activation studiesHigh-throughput screening for preclinical studies - REVPFZ (Additional S&H or Hazmat Fees May Apply)