Revvity AlphaLISA SureFire Ultra Human Phospho-SIRPα (pan-Tyr) Detection Kit, 10,000 Assay Points

Signal regulatory protein alpha (SIRPα) is a transmembrane protein expressed on myeloid cells that regulates immune responses. When bound to CD47, SIRPα recruits phosphatases (SHP-1, SHP-2), suppressing phagocytosis. Cancer cells exploit this pathway to evade immune surveillance, while dysregulation is linked to autoimmune and infectious diseases.

The AlphaLISA SureFire Ultra Human Phospho-SIRPα (pan-Tyr) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-SIRPα in cellular lysates, using Alpha Technology.

Formats:

The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

Cell and tissue lysatesAntibody modulatorsBiotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

Cellular kinase assaysReceptor activation studiesHigh-throughput screening for preclinical studies - REVPFZ (Additional S&H or Hazmat Fees May Apply)