Revvity AlphaLISA SureFire Ultra Mouse Phospho-STAT6 (Tyr641) Detection Kit, 10,000 Assay Points

Signal Transducer and Activator of Transcription 6 (STAT6) is a critical transcription factor in the JAK/STAT signaling pathway, primarily activated by interleukin-4 (IL-4) and interleukin-13 (IL-13). Upon activation, STAT6 dimerizes and translocates to the nucleus, where it regulates the expression of genes involved in anti-apoptotic and T helper 2 (Th2) immune responses. Dysregulation of STAT6 drives inflammation and hypersensitivity in allergic diseases like asthma, supports tumor survival and proliferation in cancers such as lymphomas, and plays a minor role in antiviral immunity through the STING pathway.

The AlphaLISA SureFire Ultra Mouse Phospho-STAT6 (Tyr641) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-STAT6 in cellular lysates, using Alpha Technology.

Formats:

The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with:

Cell and tissue lysatesAntibody modulatorsBiotherapeutic antibodies

AlphaLISA SureFire Ultra kits can be used for:

Cellular kinase assaysReceptor activation studiesHigh-throughput screening for preclinical studies - REVPFZ (Additional S&H or Hazmat Fees May Apply)