Revvity Ccl4/Mip-1я (H) Alphalis

Revvity Formats: - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample). Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Revvity Features: - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

No-wash steps, no separation steps ELISA alternative technology Sensitive detection Broad sample compatibility Small sample volume Results in less than 3 hours Half the time of an ELISA assay

Revvity C-C Motif Chemokine 4 (CCL4), also known as Macrophage Inflammatory Protein-1beta (MIP-1ß), is a 69 amino acid chemokine signaling through the CCR5 receptor. It is a chemoattractant for natural killer cells, monocytes, and a variety of other immune cells. CCL4 is also an important regulator of macrophage migration. CCL4 is rapidly synthesized by activated T cells, B cells, and monocytes. It is also produced by neutrophils after adhesion to the laminin contained in the basement membrane of blood vessels. It is one of the major HIV-suppressive factors produced by CD8+ T-cells and has been reported to show a direct antiviral activity against HSV-1. Recombinant CCL4 also induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)