Revvity Alphalisa High Performance Human Ifnγ Detection

Interferon gamma (IFNγ) is a homodimer glycosylated cytokine that is produced predominantly by natural killer and natural killer T cells. It is critical for innate and adaptive immunity against viral, bacterial, and protozoan infections, and is involved in tumor growth. In domestic animal species, IFNγ is associated with autoinflammatory and autoimmune diseases and inhibits viral replication and bacterial infections. Genetic variation of this cytokine is also associated with the risk of aplastic anemia.

Formats

Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features

No-wash steps, no separation stepsELISA alternative technologySensitive detectionBroad sample compatibilitySmall sample volumeResults in less than 3 hoursHalf the time of an ELISA assayHuman IFN-gamma high sensitivity detection

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.