Revvity Alphalisa Surefire Ultra Human Phospho-Cyclin D1

Revvity Cyclin D1 is encoded by CCND1 and is a member of the highly conserved cyclin family, which includes Cyclin D2 and Cyclin D3. Cyclin family members are important regulators of cell cycle progression and act as mediatiors of growth factor dependent G1 to S phase progression by playing the role of allosteric regulators of CDK4/6. Cyclin D1 is an oncogene and is found to be overexpressed in many human cancers. Abnormal levels of Cyclin D1 result in dysregulated CDK4/6 activity and leads to tumorigenesis. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity The AlphaLISA SureFire Human Phospho-Cyclin D1 (Thr286) Detection Kit is a sandwich immunoassay for the quantitative detection of phospho-Cyclin D1 (Thr286) in cellular lysates, using Alpha Technology. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Formats

The HV (high volume) kit contains reagents to run 100 wells in 96-well format, using a 60 μL reaction volume.The 500-point kit contains enough reagents to run 500 wells in 384-well format, using a 20 μL reaction volume.The 10,000-point kit contains enough reagents to run 10,000 wells in 384-well format, using a 20 μL reaction volume.The 50,000-point kit contains enough reagents to run 50,000 wells in 384-well format, using a 20 μL reaction volume.

AlphaLISA SureFire Ultra kits are compatible with

Cell and tissue lysatesAntibody modulatorsBiotherapeutic antibodies

Alpha SureFire kits can be used for

Cellular kinase assaysReceptor activation studiesScreening