Revvity Il-1 (H) Alphalisa Det.K

Revvity Formats: - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Revvity Features: - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

No-wash steps, no separation stepsELISA alternative technologySensitive detectionBroad sample compatibilitySmall sample volumeResults in less than 3 hoursHalf the time of an ELISA assay

Revvity Interleukin 1a (IL1a) is an 18 kDa pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. The IL1 family includes the agonists IL1a and IL1ß and the antagonist IL1RA. These three molecules compete for the binding to type 1 IL1 receptor (IL-1R1). IL1a is produced as a pro-protein by macrophages and monocytes, and is processed to its active form as a response to cell injury, inducing cell apoptosis. IL1a gene polymorphism has been associated with Alzheimer’s disease and rheumatoid arthritis. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)

Revvity AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. - REVPFZ (Additional S&H Or Hazmat Fees May Apply)