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Sigma-Aldrich Product Name IIC9 (CH0), 00030814 biological source hamster embryo growth mode Adheren
List Price
$1,060.00
Your Price
$1,060.00
Sigma-Aldrich Product Name IIC9 (CH0), 00030814 biological source hamster embryo growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors PAR-1 - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART:
SIALUPENN-00030814-1VL
MFG.PART:
00030814-1VL
UNSPSC:
41122409
Manufacturer:
Sigma-Aldrich
Product Name
IIC9 (CH0), 00030814
biological source
hamster embryo
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
PAR-1 (protease-activated receptor 1, thrombin receptor), EGF, PDGF
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Chinese hamster embryo fibroblast
Cell Line Description
The Chinese hamster embryo fibroblast (CHEF) line is a nontumorigenic and stable diploid cell line, with a high serum requirement for growth. It is isolated from embryo preparation and is a spontaneous tumorigenic anchorage-independent line.[1][2]
Application
IIC9 (CH0) is suitable for selective growth of some rodent epithelial cells in a medium containing citrulline.[3]
Culture Medium
DMEM:F12 (1:1) (D8062) + 5% FBS / FCS (F2442) + 2mM L-Glutamine (G7513).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:10 ie. seeding at 2-4 x10,000 cells/cm2 using 0.25% trypsin /EDTA; 37°C; 5% CO2.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-00030814-1VL |
|---|---|
| Supplier Part Number | 00030814-1VL |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |