Sigma-Aldrich Product Name 3T3-F442A, 00070654 biological source mouse adipose tissue growth mode

Mouse pre-adipocytes

Cells will differentiate into adipocytes once confluent which takes approximately 10 days. Once confluent, cells should be grown in DMEM and 10% FCS and 5 micrograms/ml insulin. Media changes should take place every 48 h.

To manage customer expectations regarding the potential of 3T3 cell line stocks to differentiate into adipocytes, if using the cells for adipocyte differentiation please note: when cells are stimulated, using an appropriate protocol, differentiation may take several weeks to occur, e.g., 2 - 5 weeks, and the proportion of the population that differentiates can be limited. If 3T3 cells from an alternate source were previously used, we cannot guarantee the differentiation performance will be the same.We are working to source a new stock of this cell line that has a higher rate of adipocyte differentiation potential which we aim to be able to offer in the future. When this is available we will update the cell line details on the website.

Once terminally differentiated the cells can be used as a model for either adipocyte differentiation or mature adipocytes.

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% Newborn Calf Serum(NBCS) (N4637)

Split sub-confluent cultures (70-80%) 1:3-1:5 ie. seeding at 2-4 x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA, 5% CO₂, 37°C. If cells are allowed to become confluent they will differentiate into adipocytes. If cryopreserving these cells, use New

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