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Sigma-Aldrich ImmunoDon BALB/c mice Myeloma Ag876 Reactivity Anti-Osteoclasts (human foetal), ECV304
List Price $436.00 Your Price $436.00
Sigma-Aldrich ImmunoDon BALB/c mice Myeloma Ag876 Reactivity Anti-Osteoclasts (human foetal), ECV304 and U937 cells technique(s) cell culture | mammalian: suitable isotype IgM plus IgG shipped in - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-01080924-DNA-5UG
MFG.PART: 01080924-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
ImmunoDon
BALB/c mice
Myeloma
Ag876
Reactivity
Anti-Osteoclasts (human foetal), ECV304 and U937 cells
technique(s)
cell culture | mammalian: suitable
isotype
IgM plus IgG
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Anti-Osteoclastoma Hybridoma
Cell Line Description
135B hybridoma was generated by fusing Ag876 myeloma cells with splenic cells of BALB/c mice immunised with tartrate-resistant acid phosphatase (TRAP) isolated from human osteoclastoma tumour. V35B antibody shows reactivity against the monocytic leukemia cell line U937, ECV304 cells and guinea pig tissue.
Immunogen
Osteoclastoma tumour
Application
Immunocytochemistry
Culture Medium
RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10% FBS (F2442) + HT Supplement (H0137)
Subculture Routine
Allow hybridomas to become 75% confluent prior to splitting. Seed at 3-9 x 100,000 cells/ml 5% CO2, 37°C,
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-01080924-DNA-5UG |
|---|---|
| Supplier Part Number | 01080924-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |