Sigma-Aldrich Product Name COS-1-InVitrus, from African green monkey kidney, 04092902, Convention on

Monkey African green kidney, SV40 transformed, serum-free

COS-1 cell line (Sigma catalog no. 88031701) adapted to grow in the chemically defined protein- and peptide-free InVitrus medium (Cell Culture Technologies). This cell line is substrate dependent, i.e., adherent. COS-1 was derived from CV-1 simian cells transformed by origin-defective mutant of SV40 which codes for wildtype T Ag. Possible host for propagation of pure populations of recombinant SV40 virus. The cells should be handled under laboratory containment level 2.

InVitrus medium (Cell Culture Technologies http://www.cellculture.com/) + 4 mM L-Glutamine (G7513) + 5 ug/ml Insulin. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO (D2650).

Split semi-confluent cultures 1:3, i.e., seeding at 4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Please note: As the cell culture medium does not contain protein it will not inactivate trypsin. Once cells have detached from the flask it is necessary to dilute the trypsin with the cell culture medium, centrifuge the cells and resuspend in fresh medium. In order to prevent syncitia formation, do not allow cells to become confluent.

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