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Sigma-Aldrich Product Name 22Rv1, 05092802 biological source human prostate growth mode Adherent
List Price $1,060.00 Your Price $1,060.00
Sigma-Aldrich Product Name 22Rv1, 05092802 biological source human prostate growth mode Adherent aggregates karyotype - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-05092802-1VL
MFG.PART: 05092802-1VL
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
22Rv1, 05092802
biological source
human prostate
growth mode
Adherent aggregates
karyotype
49,XY,del(1)(p10),+i(1)(q10),der(2)t(2;4)(p13;q31)del(2)(q13q33),der(4)t(2;4)(p13;q31),t(6;14)(q15;q32),+7,+12[5]/50,idem,+3[1]
morphology
Epithelial
products
Not specified
receptors
androgen receptor positive
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Cell Line Origin
Human Xenograft Prostate
Cell Line Description
22Rv1 is a human prostate carcinoma epithelial cell line derived from a xenograft that was serially propagated in mice after castration-induced regression and relapse of the parental, androgen-dependent CWR22 xenograft. The cell line expresses prostate specific antigen (PSA). Growth is weakly stimulated by dihydroxytestosterone and lysates are immunoreactive with androgen receptor antibody by Western blot analysis. Growth is stimulated by epidermal growth factor (EGF) but is not inhibited by transforming growth factor β-1 (TGF β-1). This cell line is tumorigenic in nude mice.
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,12
D16S539: 12
D5S818: 11,12
D7S820: 10,11
THO1: 6,9.3
TPOX: 8
vWA: 15,21
CSF1PO: 10,11
D13S317: 9,12
D16S539: 12
D5S818: 11,12
D7S820: 10,11
THO1: 6,9.3
TPOX: 8
vWA: 15,21
Culture Medium
RPMI 1640 without phenol red (Sigma R7509) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-05092802-1VL |
|---|---|
| Supplier Part Number | 05092802-1VL |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |