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Sigma-Aldrich Product Name VCaP Cell Line human, 06020201, Prostate Cancer Metastasis biological sou
List Price $436.00 Your Price $436.00
Sigma-Aldrich Product Name VCaP Cell Line human, 06020201, Prostate Cancer Metastasis biological source human prostate (metastasis) growth mode Adherent karyotype Refer to reference (Korenchuk et - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-06020201-DNA-5UG
MFG.PART: 06020201-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
VCaP Cell Line human, 06020201, Prostate Cancer Metastasis
biological source
human prostate (metastasis)
growth mode
Adherent
karyotype
Refer to reference (Korenchuk et al., 2001)
morphology
Epithelial
products
Not specified
receptors
Androgen receptor
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
Cell Line Origin
Human Prostate Cancer Metastasis
Cell Line Description
This Vertebral-Cancer of the Prostate (VCaP) cell line was established from prostate cancer tissue harvested from a metastatic lesion to a lumbar vertebral body of a patient with hormone refractory prostate cancer. The cells are reported to express large quantities of prostate specific antigen (PSA). This cell line also expresses prostatic acid phosphatase (PAP), cytokeratin-18 and the androgen receptor, and is androgen sensitive in vitro and in vivo. This cell line offers a cell-based model system of human prostate cancer. The cells are tumourigenic in SCID mice.
DNA Profile
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8,11
vWA: 18,19
CSF1PO: 10,12
D13S317: 11,12
D16S539: 9
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8,11
vWA: 18,19
Culture Medium
DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. Pellet cells by centrifugation and remove trypsin after inactivation of trypsin at each subculture.
Other Notes
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| SKU | SIALUPENN-06020201-DNA-5UG |
|---|---|
| Supplier Part Number | 06020201-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |