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Sigma-Aldrich Product Name SNL 76/7 Cell Line from mouse, 07032801 biological source mouse esophagus
List Price
$436.00
Your Price
$436.00
Sigma-Aldrich Product Name SNL 76/7 Cell Line from mouse, 07032801 biological source mouse esophagus description Mouse SIM strain embryonic fibroblast growth mode Adherent karyotype Not specified - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART:
SIALUPENN-07032801-DNA-5UG
MFG.PART:
07032801-DNA-5UG
UNSPSC:
41122409
Manufacturer:
Sigma-Aldrich
Product Name
SNL 76/7 Cell Line from mouse, 07032801
biological source
mouse esophagus
description
Mouse SIM strain embryonic fibroblast
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
Cell Line Origin
Mouse SIM strain embryonic fibroblast
Cell Line Description
SNL 76/7 clonally-derived from a STO cell line that expresses both G418 resistance and leukaemic inhibitory factor (LIF). The STO cell line was transfected with a G418-resistance cassette, RV4.0 (Thomas and Capecchi 1987), and a LIF expression construct (Williams et al., 1988). This cell line can be used as a feeder layer to support the growth of mouse embryonic stem cells. Inclusion of G418 in the media is not necessary for normal cell growth.
Culture Medium
Knockout D-MEM (Invitrogen) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Split confluent cultures 1:5-1:15 i.e. seeding at 2-4x10,000 cells/cm2 using trypsin/EDTA;5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-07032801-DNA-5UG |
|---|---|
| Supplier Part Number | 07032801-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |