Sigma-Aldrich Product Name PNT2 (SERUM FREE), 07042701 biological source human prostate growth mode

Human prostate normal, serum-free

This cell line is a derivative of the normal human prostate cell line PNT2 (Sigma cat no. 95012613) adapted to grow in serum-free medium. PNT2 was established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from a prostate of a 33 year old male at post mortem. PNT2 cells contain the SV40 genome and express large T protein.

Defined Keratinocyte-SFM (Gibco 10785) + Growth Supplement (Gibco 10784-015).

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Use Trypsin Inhibitor to inactivate the trypsin. When freezing cells down use conditioned medium plus 10% DMSO.

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