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Sigma-Aldrich Product Name NS20Y-BU-4, 08062503 biological source mouse nerve growth mode Adherent
List Price $436.00 Your Price $436.00
Sigma-Aldrich Product Name NS20Y-BU-4, 08062503 biological source mouse nerve growth mode Adherent karyotype Not specified morphology Neuronal products Choline acetyltransferase receptors Not - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-08062503-DNA-5UG
MFG.PART: 08062503-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
NS20Y-BU-4, 08062503
biological source
mouse nerve
growth mode
Adherent
karyotype
Not specified
morphology
Neuronal
products
Choline acetyltransferase
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Related Categories
Cell Culture & Analysis
Cell Lines & Specialty Cell Culture
Mammalian Cell Lines
Molecular Biology & Functional Genomics
Cell Line Origin
Mouse neuroblastoma
Cell Line Description
A bromodeoxyuridine-resistant mutant of NS20Y (Sigma Catalogue number 08062517) The cell line NS20Y is a cholinergic cell line derived from mouse neuroblastoma C1300 Mouse strain A/Jax.
Application
Used in the generation of cell hybrids.
Culture Medium
DMEM + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)+ 1 mM 8-bromodeoxyuridine. Cells can also be grown in the absence of bromodeoxyuridine
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.05% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-08062503-DNA-5UG |
|---|---|
| Supplier Part Number | 08062503-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |