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Sigma-Aldrich Product Name h1RPE7, from human eye, 09061602 biological source human eye growth mode
List Price $436.00 Your Price $436.00
Sigma-Aldrich Product Name h1RPE7, from human eye, 09061602 biological source human eye growth mode Adherent karyotype Not specified morphology Polygonal products Cytokeratins 5 and 8, junctional - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-09061602-DNA-5UG
MFG.PART: 09061602-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
h1RPE7, from human eye, 09061602
biological source
human eye
growth mode
Adherent
karyotype
Not specified
morphology
Polygonal
products
Cytokeratins 5 and 8, junctional protein zonula occludens (ZO-1)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
Cell Line Origin
An immortalized retinal pigment epithelial cell line
Cell Line Description
The h1RPE7 cell line was derived from primary human retinal pigment epithelial cells obtained from a 50-year-old female Caucasian donor. The primary culture was transfected with a construct encoding SV40 large T antigen that was deleted for the small t antigen along with puromycin resistance gene.
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 10,13
D13S317: 12
D16S539: 12
D5S818: 9,13
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 16,19
Amelogenin: X
CSF1PO: 10,13
D13S317: 12
D16S539: 12
D5S818: 9,13
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 16,19
Culture Medium
Hams F10 + 20% Heat-inactivated FCS + 2 mM Glutamine + selection antibiotic 1μg/ml puromycin.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. Population doubling approx 24hrs.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
| SKU | SIALUPENN-09061602-DNA-5UG |
|---|---|
| Supplier Part Number | 09061602-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |