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Sigma-Aldrich Product Name Mero-14, 09100101 biological source human lung growth mode Adherent
List Price $1,060.00 Your Price $1,060.00
Sigma-Aldrich Product Name Mero-14, 09100101 biological source human lung growth mode Adherent karyotype Number of chromosomes 72-75 morphology Spindle shaped cells products Cytokeratins 7, 8, 18 - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-09100101-1VL
MFG.PART: 09100101-1VL
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
Mero-14, 09100101
biological source
human lung
growth mode
Adherent
karyotype
Number of chromosomes 72-75
morphology
Spindle shaped cells
products
Cytokeratins 7, 8, 18 and 19. Epithelial membrane antigen (EMA)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Cell Line Origin
A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure
Cell Line Description
Mero-14 was derived from the pleural effusion from a male patient aged >60 with a history of asbestos exposure. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Application
Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 12,13
D5S818: 9
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,18
CSF1PO: 10
D13S317: 12
D16S539: 12,13
D5S818: 9
D7S820: 10,11
THO1: 8
TPOX: 9,11
vWA: 15,18
Culture Medium
Hams F10 + 15% FCS + 2mM Glutamine
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 24hrs
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-09100101-1VL |
|---|---|
| Supplier Part Number | 09100101-1VL |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |