Sigma-Aldrich Product Name MCC14/2 cell line human, 10092303 biological source human skin descriptio

Human Merkel carcinoma

Merkel cell carcinoma cell line. Cell Bank Australia reference number: CBA1340.

RPMI 1640 (with 2 mM L-Glutamine + 25 mM HEPES) + 15% FCS/FBS

Optimal split ratio 1:8 (seeding density 10⁴ cells/cm²).Cells incubated at 37 °C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min.

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Depositor and originator: Helen Leonard - Queensland Institute of Medical Research, Australia