Sigma-Aldrich Product Name MM386 cell line human, 10092318 biological source human skin growth mode

Human malignant melanoma

Malignant Melanoma. Cell Bank Australia reference number: CBA1349.

Not specified

RPMI 1640 (with 2 mM L-Glutamine + 25 mM HEPES) +10% FBS.

Optimal split ratio 1:4 (seeding density 1.2 x10⁴ cells/cm²).Cells incubated at 37 °C with 5% CO₂.Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min.

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Peter Parsons - Queensland Institute of Medical Research, Australia