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Sigma-Aldrich Product Name P4E6 cell line, biological source human prostate Quality Level 100 growth
List Price
$1,060.00
Your Price
$1,060.00
Sigma-Aldrich Product Name P4E6 cell line, biological source human prostate Quality Level 100 growth mode Not specified karyotype Not specified morphology Epithelial products Stains positive for - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART:
SIALUPENN-10112301-1VL
MFG.PART:
10112301-1VL
UNSPSC:
41122409
Manufacturer:
Sigma-Aldrich
Product Name
P4E6 cell line,
biological source
human prostate
Quality Level
100
growth mode
Not specified
karyotype
Not specified
morphology
Epithelial
products
Stains positive for pancytokeratin, cytokeratin 8, vimentin (weak) and chromogranin A. Prostate markers expressed include prostate specific antigen (PSA) prostate specific membrane antigen (PSMA), negative for expression of androgen receptor (AR).
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
Related Categories
Cell Culture & Analysis
Cell Lines & Specialty Cell Culture
Mammalian Cell Lines
Molecular Biology & Functional Genomics
Cell Line Origin
Prostate cancer well differentiated, early stage
DNA Profile
STR-PCR Data:
Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 7,8
THO1: 7,9.3
TPOX: 8
vWA: 17,19
Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 11
D5S818: 12
D7S820: 7,8
THO1: 7,9.3
TPOX: 8
vWA: 17,19
Culture Medium
Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine + 2% Fetal Bovine Serum (FBS). Addition of 2% serum was found to increase cell viability.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor (the culture medium contains too low a serum content to inactivate the trypsin). To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form. Additionally, any recipient wanting to use the cell line in the field of vaccines for the treatment or prevention of prostate cancer should first obtain permission from GemVax & KAEL Co., Ltd. Contact : Dr. Eujin Hyun, Head of R&D Department (eujinhyun@kaelgemvax.com).
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
| SKU | SIALUPENN-10112301-1VL |
|---|---|
| Supplier Part Number | 10112301-1VL |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |