Sigma-Aldrich Product Name LIM2551, 12070505 biological source human colon growth mode Semi-adherent

Human derived from an adenocarcinoma of the transverse colon

LIM2551 was derived from an adenocarcinoma of the transverse colon. Cell Bank Australia reference number: CBA0170.

Not specified

RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + FBS / FCS (F2442), Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10 μM.

Optimal split ratio 1:8-1:16 (seeding density 1 x 10⁴ cells/cm²). Cells incubated at 37 °C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. LIM2551 requires the growth medium to be changed 3 times each week. Feed and passage as for semi-adherent culture (collect medium with floating cells and then trypsinise the adherent population, pool both together before reseeding).

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Depositor and originator: Professor Tony Burgess - Ludwig Institute for Cancer Research Ltd, Melbourne, Australia