Sigma-Aldrich PET-17B DNA 1SET

The pET-17b vector carries an N-terminal 11aa T7-Tag^® sequence followed by a region of useful cloning sites. Included in the multiple cloning region are dual BstX I sites, which allow efficient cloning using an asymmetric linker (1). Unique sites (except for the two BstX I sites) are shown on the vector map (TB047). Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map.

The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.

Toxicity: Standard Handling

Seed, B. (1987) Nature 329, 840.

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

T7-Tag is a registered trademark of Merck KGaA, Darmstadt, Germany

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