Sigma-Aldrich PET-32 XA/LIC VECTOR KIT 1SET

The pET-32 Xa/LIC vector is an Xa/LIC version of pET-32b(+). It is designed for cloning and high-level expression of target proteins fused with the 109aa Trx•Tag thioredoxin protein, and His•Tag and STag coding sequences that are cleavable with Factor Xa protease. The plasmid contains a strong T7lac promoter, an optimized RBS, the coding sequence for the Xa protease cleavage site (IleGluGlyArg↓), and a multiple cloning site that contains restriction enzyme sites found in many other Novagen expression vectors to facilitate insert transfer. An optional C-terminal His•Tag coding sequence is compatible with purification, detection, and quantification...




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•1 µgXa/LIC Vector

•10 µlXa/LIC Control Insert

•25 UT4 DNA Polymerase, LIC-qualified

•50 µl10X T4 DNA Polymerase Buffer

•100 µl100 mM DTT

•50 µl25 mM EDTA

•40 µl25 mM dGTP

•1.5 mlNuclease-free Water

•22 × 50 µlNovaBlue GigaSingles Competent Cells

•0.2 mlBL21(DE3) Competent Cells

•0.2 mlBL21(DE3)pLysS Competent Cells

•5 × 2 mlSOC Medium

•10 µlTest Plasmid

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

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