We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn about our cookie policy.
Sigma-Aldrich Product Name SV3T3B, 85011409 biological source mouse embryo growth mode Adherent
List Price $436.00 Your Price $436.00
Sigma-Aldrich Product Name SV3T3B, 85011409 biological source mouse embryo growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-85011409-DNA-5UG
MFG.PART: 85011409-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
SV3T3B, 85011409
biological source
mouse embryo
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse SIM embryo fibroblast
Cell Line Description
A 3T3 cell line doubly transformed by polyoma then SV40 viruses, probably the fore-runner of SV-T2 (Sigma Catalogue number. 90030801). Demonstrates a high degree of contact inhibition.
Application
Contact inhibition, transformation and virus studies: SV40
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-85011409-DNA-5UG |
|---|---|
| Supplier Part Number | 85011409-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |