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Sigma-Aldrich Product Name BHK TK-, 85011423 biological source hamster kidney growth mode Adherent
List Price $436.00 Your Price $436.00
Sigma-Aldrich Product Name BHK TK-, 85011423 biological source hamster kidney growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-85011423-DNA-5UG
MFG.PART: 85011423-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
BHK TK-, 85011423
biological source
hamster kidney
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Hamster Syrian kidney
Cell Line Description
BHK TK- is a derivative of BHK21 (clone 13) rendered TK- by selection in 30μg/ml BUdR. Used widely for research on viruses and on neoplastic transformation especially with polyoma virus.
Application
Transformation and virus studies
Culture Medium
DMEM or GMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-85011423-DNA-5UG |
|---|---|
| Supplier Part Number | 85011423-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |