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Sigma-Aldrich Product Name LTK-, 85011432 biological source mouse connective tissue growth mode
List Price
$436.00
Your Price
$436.00
Sigma-Aldrich Product Name LTK-, 85011432 biological source mouse connective tissue growth mode Adherent karyotype Not specified morphology Fibroblast products Not specified receptors Not specified - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART:
SIALUPENN-85011432-DNA-5UG
MFG.PART:
85011432-DNA-5UG
UNSPSC:
41122409
Manufacturer:
Sigma-Aldrich
Product Name
LTK-, 85011432
biological source
mouse connective tissue
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse C34/An connective tissue
Cell Line Description
Sub-line of BUdR resistant strain of the L-M mouse line (Sigma Catalogue number. 87032401), which in turn was derived from L929 (Sigma Catalogue number. 85011425). TK- cells cannot grow in HAT medium. Stable TK- mutants are only maintained if the cells are kept for several passages in growth medium containing BUdR.
Application
Somatic cell hybridisation and gene transfer studies
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split semi-confluent cultures seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-85011432-DNA-5UG |
|---|---|
| Supplier Part Number | 85011432-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |