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Sigma-Aldrich Product Name C57/B1, 85011438 biological source mouse skin growth mode Adherent karyot
List Price $436.00 Your Price $436.00
Sigma-Aldrich Product Name C57/B1, 85011438 biological source mouse skin growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors Not specified - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-85011438-DNA-5UG
MFG.PART: 85011438-DNA-5UG
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
C57/B1, 85011438
biological source
mouse skin
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Cell Line Origin
Mouse C57BL/6J melanoma epithelial
Cell Line Description
Spontaneous tumour of C57 black/6J mouse originally obtained from Dr Parrish of Australian National University, Canberra. Rendered HGPRT- by selection in 20μg/ml 6-thioguanine.
Application
Tumourigenicity studies
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) at a maximum dilution of 1:10 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Monolayers will detach if monolayer becomes over-confluent.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-85011438-DNA-5UG |
|---|---|
| Supplier Part Number | 85011438-DNA-5UG |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |