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Sigma-Aldrich Product Name Bu25 TK-, 85022104 biological source human cervix growth mode Adherent
List Price $1,060.00 Your Price $1,060.00
Sigma-Aldrich Product Name Bu25 TK-, 85022104 biological source human cervix growth mode Adherent karyotype Not specified morphology Epithelial products Not specified receptors Not specified - SIALUPENN (Additional S&H or Hazmat Fees may Apply)
NETA PART: SIALUPENN-85022104-1VL
MFG.PART: 85022104-1VL
UNSPSC: 41122409
Manufacturer: Sigma-Aldrich
Product Name
Bu25 TK-, 85022104
biological source
human cervix
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
Related Categories
Cell Culture & Analysis
Cell Lines & Specialty Cell Culture
Mammalian Cell Lines
Molecular Biology & Functional Genomics
Cell Line Origin
Human cervical carcinoma
Cell Line Description
HeLa derivative rendered TK- by selection in BUdR.
Application
Transfection studies
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) seeding at 4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA. 5% CO2; 37°C. Passage every 2-3 days when confluent at 1:3 dilution.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
| SKU | SIALUPENN-85022104-1VL |
|---|---|
| Supplier Part Number | 85022104-1VL |
| UM | EA |
| UNSPSC | 41122409 |
| Manufacturer | Sigma-Aldrich |
| ProductLine | SIALUPENN |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 9 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |